Ulex europaeus (Gorse) Lectin (UEA I) - Pure

Ulex europaeus (Gorse) Lectin (UEA I) - Pure

Regular price $169.00 Sale
SKU# 21510130-1

Product Description

Ulex europaeus agglutinin (UEA I) is isolated from gorse seeds and separated from crude extract. It has a molecular weight of 63,000 that consists of two subunits and has an isoelectric point between pH 4.5 and pH 5.1. The lectin is a glycoprotein that has no mitogenic activity and agglutinates type O erythrocytes, and of a lesser degree to individuals with A2 erythrocytes.

UEA I has a carbohydrate specificity for L-fucose and elutes with the sugar L-fucose. The lectin reacts strongly with ?(1,2) linked fucose residues but poorly or not at all with ?(1,3) or ?(1,6)-linked fucose. UEA I is unable to bind internal fucose structures and the presence of an internal ?(1,3)-linked fucose on the H type 2 oligosaccharide decreases lectin affinity for this blood group antigen by 3-fold.

UEA I has been used as a marker for vascular endothelium and used to identify endothelial cells of benign vascular lesions more specifically than antibodies of Factor VII-related antigen. UEA I coated magnetic beads have been used to isolate endothelial cells associated with micro-vessels for subsequent cell culture. This lectin has also been used to differentiate between angiosarcomas and epithelial tumors.

This product comes in a lyophilized form and is stored at -20°C. Calcium, manganese, and zinc ions are required for binding.

Technical Specifications

Molecular Weight: 63 kDa
Synonyms: UEA I
Shelf Life: 2 years
Storage Temperature: -20°C
Hazardous Shipping: Non-hazardous