Rubisco (RbcL) recombinant protein standard (250µl)
PEB extraction buffer (3 x 2 ml) (4X stock)
Storage (Antibody and Standard)
lyophilized/reconstituted at -20°C (6 months) or -80°C (long term storage/years). Please avoid freezing and thawing of reconstituted antibodies. Make aliquots instead
Stable at RT for at least 1 month; (short term storage/month) at 4°C or long term storage (1 year) at 20°C.
The rubisco quantitation kit is suitable for quantitation of Rubisco in plant and algal samples using quantitative immunoblotting. The kit contains anti-RbcL antibody, recombinant RbcL protein and PEB buffer for quantitative protein extraction.
Predicted reactivity:di and monocots, conifers, mosses, liverworts, welwitschia, green algae, red alge, brown algae, cryptomonad, cyanobacteria including prochlorophytes, gamma-proeobacteria, beta-proteobacteria, alpha proteobacteria
Not reactive in: no confirmed exceptin from predicted reactivity known in the moment
RbcL protein standard: at 0.15 pmoles/µl, amount enough for generation of standard curve in 85 assays (standard curve: 0.0625 pmoles, 0.125 pmoles, 0.25 pmoles)
PEB extraction buffer: amount enought for 48 isolations of plant material (using 500µl 1 x PEB for 100 mg fresh weight) or 120 isolations of algal material (using 200µl 1 x PEB for cell amounts corresponding to 4-10 ug total chlorophyll)