Protoplast Regeneration Medium
IgGPure Protein A Separopore® Kit consists of affinity matrix with immobilized Protein-A (from Staphylococcus aureus) for purification and/or fractionation of IgG subclasses, fractionation of IgG fragments, fractionation of cells tagged with IgG, and isolation of antigen-antibody complexes. In addition, Protein A interacts with some classes of IgM and IgA. IgGPure Protein A-Separopore® kit is reliable tool to purify monoclonal and polyclonal IgG from ascies, serum and cell culture supernatants. Protein A Agarose can bind >18 mg of human IgG/ml of drained gel.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
- Binding/Wash Buffer: 100 mL (10X)
- IgG Elution buffer: 50 ml (10X)
- Neutralization buffer: 20 ml