Phaseolus vulgaris Lectin (PHA-E+L) - Pure
Phaseolus vulgaris lectin (PHA E+L, also known as PHA P, P standing for phytohemagglutinin) is isolated from red kidney beans. It consists of two isolectins PHA-E and PHA-L, erythroagglutinin and leucoagglutinin, of which both possess strong mitogenic activity. PHA P has a molecular weight of 126,000 and an isoelectric point between pH 5.2 and pH 6.2. It has a carbohydrate specificity for complex oligosaccharides and elutes with bovine thyroglobulin or acetic acid. This lectin agglutinates blood group A (-SA).
PHA P can be used for mitogenic stimulation to human lymphocytes. It has the potential to induce closer contacts between adjacent cell membranes and has been successfully used for membrane-induced fusion in human oocytes, bovine oocyt, and caprine oocytes. Phytohemagglutinin was direct evidence for the involvement of bacterial lectins in the initiation of infection, the basis for present attempts in academia and industry to apply carbohydrates for anti-adhesion therapy of such diseases.
This product comes in a lyophilized form and is stable for more than three years when stored below -20ºC. Calcium and manganese ions are required for binding activity. This lectin is not soluble in distilled water and dilute buffers containing 10 - 15 mM NaCl solubilize it.
|Molecular Weight||:||126 kDa|
|Shelf Life||:||2 years|