Isopropyl-‚âà√≠‚Äö√¢¬ß-D-Thiogalactopyranoside, an inducer of‚âà√≠‚Äö√¢¬ß-galactosidase is used to promote the expression of proteins in cells controlled by the lac operator system.
Supplied as a ready-to-use solution to be used with X-gal for blue white colony screening.
- Petriplates: Use 40‚âà√≠ºlper Petriplates (9 cm diameter).
- LB agar: Use 1 ®µl/mL of liquid LB agar medium just before pouring (~50®‚Äö√†¬¥C).
- Liquid culture: Use 1®µl/mL of liquid culture medium.