HEPES Buffer 1M, pH 7.3

HEPES Buffer 1M, pH 7.3

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SKU# 40820058-1

Product Description

HEPES, also referred to as 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, is an N-substituted aminosulfonic acid buffer with an optimal buffering pH range of 6.0 to 8.5. HEPES, when applied as a separator in ultrathin isoelectric focusing gels, enhances phosphoglucomutase resolution. HEPES is zwitterionic at most biological pHs making it one of the best all-purpose buffers available for use in biological research. It is also catogorized under Good's buffers. HEPES may be used in isoelectric focusing applications and as a buffer in the quantitative and selective measurement of antigen-antibody reactions. HEPES is reportedly superior to NaHCO in controlling pH in tissue and organ culture. Not recommended for use with the Folin-Ciocalteu protein assay.

Technical Specifications

CAS#: 7365-45-9
Molecular Weight: 238.3
Molecular Formula: C8H18N2O4S
Synonyms: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid
Appearance Form: Liquid
Appearance Color: Clear
Concentration: 1.0M
Working Concentration: 0
pH: 7.3
Solubility: Soluble in water
Shelf Life: 4 years
Storage Temperature: 2-8°C
Hazardous Shipping: Non-hazardous
HTS Code: 3822.00.0002

Applications

References