Griffonia simplicifolia Lectin (GSL I) - Pure

Griffonia simplicifolia Lectin (GSL I) - Pure

Regular price $131.99 Sale
SKU# 21511060-1

Product Description

Griffonia simplicifolia lectin (GSL I) is isolated from a woody climbing shrub native to Africa and purified by affinity chromatography. GSL I has a molecular weight of 114,000 and an isoelectric point between pH 5.0 and pH 6.5. GSL I is composed of two types of subunits A and B that combine to form tetrameric structures, resulting in five isolectins. This lectin has affinity for terminal ?-D-galactosyl residues and terminal N-acetyl-?-D-galactosaminyl residues, and elutes with the sugar galactose. The A-rich lectin preferentially agglutinates blood group A erythrocytes and thus appears to be specific for ?-N-acetylgalactosamine residues, while the B-rich lectin preferentially agglutinates blood group B cells and is specific for ?-galactose residues. This lectin shows specificity for blood group B greater than blood group A1.

GSL I has been reported to bind several glycoproteins including laminin. Studies show that some ?-D-galactosyl residues are expressed in endothelial cells of mouse tissues and provide evidence that these cells can present a highly specific surface of glycosylation pattern.

This product comes in lyophilized form and is stored at -20ºC. Reconstitute with sterile buffer. Calcium and manganese ions are required for binding.

Technical Specifications

Molecular Weight: 114 kDa
Synonyms: GSL I
Shelf Life: 2 years
Storage Temperature: -20°C
Hazardous Shipping: Non-hazardous