Glutathione Separopore® 4B-CL

Product Description

Applications:

Reduced glutathione is covalently linked Separopore^® 4B for use in affinity purification of glutathione-S-transferase (GST) and GST-fused proteins. Glutathione Separopore^® Super Flow (SF) is used for protein purification by batch/column and scale-up procedures. Glutathione Separopore^® Super Flow (SF) has an exellent protein binding capacity. The good flow properties of the 92 μm (average size) highly crosslinked Separopore^® matrix makes an excellent choice for protein purification by column purification or batch process for scale-up.

This product provides a one step purification method and permits rapid, mild and highly selective purifications of proteins containing glutathione binding sequences. Glutathione-Separopore is prepared by covalently coupling glutathione to epoxy-activated 4% crosslinked agarose beads to form a stable thioether linkage. The coupling was optimized to give a high binding capacity of 5 mg or more of Glutathione-S-transferase (GST) per ml of wet gel. Bound GST–fusion proteins are easily displaced from the resin by elution with buffers containing reduced glutathione. Mild elution conditions preserve protein antigenicity and function.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

Technical specifications:

• Storage: 2 °C – 8 °C