Glutathione Separopore® 4B
Reduced glutathione is covalently linked Separopore¬Æ 4B for use in affinity purification of glutathione-S-transferase (GST) and GST-fused proteins. Glutathione Separopore¬Æ 4B provides a one step purification method and permits rapid, mild and highly selective purifications of proteins containing glutathione binding sequences. Glutathione Separopore¬Æ 4B is prepared by covalently coupling glutathione to epoxy-activated 4 % crosslinked agarose beads to form a stable thioether linkage. The coupling was optimized to give a high binding capacity of 5 mg or more of Glutathione-S-transferase (GST) per ml of wet gel.. Bound GST -fusion proteins are easily displaced from the resin by elution with buffers containing reduced glutathione. Mild elution conditions preserve protein antigenicity and function.
Note: Separopore¬Æ is a cost-effective equivalent to Sepharose¬Æ in all of its physical properties and binding characteristics.