Agaricus bisporus Lectin (ABA/ABL) - Pure

Agaricus bisporus Lectin (ABA/ABL) - Pure

Regular price $179.50 Sale
SKU# 21510154-1

Product Description

Agaricus bisporus agglutinin/lectin (ABA/ABL) is isolated from white button mushrooms. It has a molecular weight of 58,500 as determined by gel filtration that corresponds to a tetramer, and has an isoelectric point that ranges between pH 5.0 and pH 6.0. ABA is a mixture of two phytohemagglutinins (PHA) with similar specificities for carbohydrate (PHA-A and PHA-B). Each monomer has 2 distinct carbohydrate binding sites, one for galactose-ß≤-1,3-N-acetylgalactosamine and another for galactose-ß≤-1,3-N-acetylglucosamine, but does not bind monosaccharides.

ABA has a non-specific blood group recognition since PHA-A and PHA-B agglutinate erythrocytes independent of their blood group type. The O-linked glycopeptide released by trypsin is a potent inhibitor of both the isolectins. Removal of the terminal sialic acid residue from the glycopeptide increases its inhibitory potency by 8-fold. Periodate or ß≤-galactosidase treatment of the trypsin released glycopeptide destroys all inhibitory activity. Simple sugars are very poor inhibitors of these isolectins. However, a galactose residue appears to play a major role in these lectin binding mechanisms. The sugar linkage is also important since a synthetic N-linked glycopeptide is not inhibitory.

ABA can be internalized by clathrin-coated vesicles after binding to surface glycoproteins. After internalization, it inhibits nuclear import of nuclear localization signal-dependent proteins. See other ABA conjugates.

This product comes in lyophilized form and is stored below -20ºC.

Technical Specifications

Molecular Weight: 58.5 kDa
Synonyms: ABA/ABL
Appearance Form: White to off-white
Shelf Life: 2 years
Storage Temperature: -20°C
Hazardous Shipping: Non-hazardous