Hippeastrum hybrid Lectin (HHL) - Pure
Hippeastrum hybrid lectin (HHL) isolated from amaryllis bulbs and purified by affinity chromatography. It consists of four subunits of identical size and has a molecular weight of 50,000. HHL has an isoelectric point between pH 4.7 and pH 5.1. It only binds ?-mannose residues, (?-1,3) and (?-1,6) linked mannosyl units, as well as some yeast galactomannans, and elutes with the sugar Me?Man. HHL has a blood group specificity for rabbit.
Study shows when treatment of the linear ?-1,3-mannan with periodate, resulting in oxidation of the terminal, nonreducing mannosyl group, reactivity with HHL was not reduced. Oligosaccharides containing either 1,3- or 1,6-linked mannosyl units are good inhibitors of HHL-mannan precipitation system. Observations suggests that HHL reacts not only with terminal but also with internal ?-D-mannosyl residues. Lectin appears to possess extended binding sites complementary to at least three 1,6-linked ?-mannosyl units. Those containing ?-1,3- or ?-1,6-mannosyl residues were retarded on immobilized HHL agarose column.
This product comes in a lyophilized form and is stored at -20ºC. No metals ions are required for binding.
|Molecular Weight||:||50 kDa|
|Shelf Life||:||2 years|