Lens culinaris Lectin (LCA/LCH) - Pure

Lens culinaris Lectin (LCA/LCH) - Pure

Regular price $296.18 Sale
SKU# 21510006-1

Product Description

Lens culinaris lectin (LCA) is isolated from lentil seeds and purified by affinity chromatography. LCA is composed of four subunits that consists of an α-chain (MW: 5.7 kDa) and α-chain (MW: 17.5 kDa), with a total molecular weight of 46,000. It has an isoelectric point between pH 7.6 and pH 8.4 and a carbohydrate specificity toward α-D-mannose and α-D-glucose, eluting with the sugar MeαMan+MeαGlc. This lectin is useful in affinity chromatography columns for the separation of glycoconjugates.

The two isomers LCA-A and LCA-B agglutinates human red blood cells, although not blood group specific. Activity of LCA is determined by haemagglutination with human blood and agglutinates a 2% suspension of human erythrocytes at a lectin concentration of ?8µg/ml.

This product comes in a lyophilized form and is stable for more than three years when stored at -20ºC. Calcium and manganese ions are required for binding.

Technical Specifications

Molecular Weight: 46 kDa
Synonyms: LCA/LCH
Shelf Life: 1 year
Storage Temperature: -20°C
Hazardous Shipping: Non-hazardous

Applications

Cancer biomarker, Agglutination studies, Separation of glycoconjugates

References