Psophocarpus tetragonolobus (Winged bean) Lectin (PTL/PTA I) - Pure
Psophocarpus tetragonolobus lectin (PTL I, psophocarpin B1, a storage protein with chymotrypsin inhibitory activity), is isolated from winged bean seeds and purified by affinity chromatography. The seed contains two lectins in which PTL I is the basic of the two, with an isoelectric point greater than pH 9.5 and a molecular weight of 58,000. PTL I is a dimeric glycoprotein with specificity to GalNAc, with eluting sugar GalNAc. This lectin agglutinates trypsinized rabbit and human erythrocytes (type A and B) but not trypsinized human type O erythrocytes. PTL I recognizes blood group A-substance derived oligosaccharides such as trisaccharide GalNAc?1-3Gal?1-4Glc.
Reports on the thermodynamics and kinetics of the lectin suggest that it has an extended binding site, and its interactions with saccharides involve reorientation of water molecules.
This product comes in a lyophilized form and is stored at -20°C.
|Molecular Weight||:||58 kDa|
|Shelf Life||:||2 years|