Psophocarpus tetragonolobus (Winged bean) Lectin (PTL/PTA I+II) - Pure

Psophocarpus tetragonolobus (Winged bean) Lectin (PTL/PTA I+II) - Pure

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SKU# 21511410-1

Product Description

Product Description
Psophocarpus tetragonolobusåÊ(psophocarpinåÊB1, a storage protein with chymotrypsin inhibitory activity), PTL I lectin is isolated from winged bean seeds and purified by affinity chromatography. The seed contains two lectins in which PTL I is theåÊbasicåÊof the two, with an isoelectric point greater than pH 9.5 and a molecular weight of 58,000. PTL I is a dimeric glycoprotein with specificity to GalNAc, eluting the sugar GalNAc.

This lectin agglutinates trypsinized rabbit and human type A and B erythrocytes but not trypsinized human type O erythrocytes. PTL I recognizes blood group A-substance derived oligosaccharides such as trisaccharide GalNAcë±1-3Galë_1-4Glc. Studies on inhibition assays of lectin-blood group A precipitation demonstrated that substance-derived oligosaccharides were the best inhibitors and had the common structure of D-GalNAc alpha(1,3)D-Gal-(beta 1,3/4) to a D-Glc, and about 8 and 4 times more active than D-GalNAc and D-GalNAc alpha(1,3)D-Gal, respectively.

Reports on the thermodynamics and kinetics of the lectin suggest that it has an extended binding site, and its interactions with saccharides involveåÊreorientationåÊof water molecules.

This product comes in a lyophilized powder form and is stored at -20å¡C.åÊ

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Applications:
Glycobiology
Staining of mouse M-cells
Blood vessel staining of A and B group individuals

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References

  • Kortt, A. Purification and properties of the basic lectins from winged bean seed [Psophocarpus tetragonolobusåÊ(L.) DC].åÊFEBS J. (1984) 138(3):519-525.
  • Matsuda, T., et al. Carbohydrate binding specificity of the basic lectin from winged bean (Psophocarpus tetragonolobus).åÊMol Immunol.åÊ(1989) 26(2):189-95.
  • Patanjali, S., et al. Erythrocyte-binding studies on an acidic lectin from winged bean (Psophocarpus tetragonolobus).åÊJåÊbiochem. (1988) 252:625-631.
  • Sharma, V., et al. Cloning and sequencing of the winged bean (Psophocarpus tetragonolobus)åÊbasic agglutinin (WBA I):åÊpresenceåÊof second glycosylation site and its implications in quaternary structure.åÊFEBS J.åÊ(1996) 389:289-292.

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Technical Specifications

Molecular Weight: 58 kDa
Shelf Life: 2 years
Storage Temperature: -20°C
Hazardous Shipping: Non-hazardous

Technical Documentation