PlantGene™ Ex-Amp PCR Kit
Ex-Amp PCR Kit is ready for quick extraction and PCR amplification of genomic DNA from any plant tissue. The plant DNA is generally extracted from fresh leaf tissue 5x5mm size pieces by incubation in extraction solution for 10 min at 95C. The quenching solution is added to the supernatant to neutralize any inhibitory substances. An aliquot of this mixture can be directly used for PCR by adding Reaction mix that contains FirePol with taq antibodies Dye, buffer, dNTP and Taq polymerase. The product can be directly loaded on an agarose gel.