Glutathione Separopore® 6B-CL

Glutathione Separopore® 6B-CL

Regular price $298.00 Sale
SKU# 20182013-1

Product Description

Applications:

Reduced glutathione is covalently linked to High Resolution Separopore® 6B-CL for use in affinity purification of glutathione-S-transferase (GST) and GST-fused proteins. This product provides a one step purification method and permits rapid, mild and highly selective purifications of proteins containing glutathione binding sequences. Glutathione-Separopore is prepared by covalently coupling glutathione to epoxy-activated highly crosslinked 6% agarose beads to form a stable thioether linkage. The coupling was optimized to give a high binding capacity of 5 mg or more of Glutathione-S-transferase (GST) per ml of wet gel. Bound GST -fusion proteins are easily displaced from the resin by elution with buffers containing reduced glutathione. Mild elution conditions preserve protein antigenicity and function. Glutathione Separopore® HR ensures the lowest sample dilution and best high-resolution separation.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.