Euonymus europaeus (Spindle tree) Lectin (EEL/EEA) - Pure

Euonymus europaeus (Spindle tree) Lectin (EEL/EEA) - Pure

Regular price $79.00 Sale
SKU# 21510004-1

Product Description

Euonymus europaeus lectin or agglutinin (EEL/EEA) is isolated from the spindle tree and purified by affinity chromatography. It is a glycoprotein with a molecular weight of 140,000 and is composed with most of the subunits that appear to consist of two chains linked by disulfide bonds. EEL consists of six closely related lectins (isoforms) with isoelectric points between pH 4.3 and pH 4.7.

EEL is identified for its erythrocyte agglutinating properties and specificity for blood groups B and H. Studies have shown a stronger binding of the H trisaccharide over the B trisaccharide, but with general pattern of the lectin interacting with both B and H blood groups and ?-mannose-terminated saccharides. EEL has a preferential recognition of terminal ?-fucose residues in H and B blood group-related carbohydrates. This lectin binds to endothelial cells from human and non-human sources, and recognizes carbohydrate structures on the surface of stimulated murine peritoneal lymphoid cells.

This product comes in a lyophilized form and is stored at -20°C. Reconstitute with sterile buffer. Metal ions calcium and zinc are required for binding.

Technical Specifications

Molecular Weight: 140 kDa
Synonyms: EEL/EEA
Shelf Life: 2 years
Storage Temperature: -20°C
Hazardous Shipping: Non-hazardous