Rubisco Quantitation Kit

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SKU# 30210005-1

Product Description

Rubisco Quantitation Kit

Kit Contains:

Storage (Antibody and Standard)

lyophilized/reconstituted at -20®‚Äö√†¬¥C (6 months) or -80®‚Äö√†¬¥C (long term storage/years). Please avoid freezing and thawing of reconstituted antibodies. Make aliquots instead

Storage (Buffer)

Stable at RT for at least 1 month; (short term storage/month) at 4®‚Äö√†¬¥C or long term storage (1 year) at 20®‚Äö√†¬¥C.

Background:

The rubisco quantitation kit is suitable for quantitation of Rubisco in plant and algal samples using quantitative immunoblotting. The kit contains anti-RbcL antibody, recombinant RbcL protein and PEB buffer for quantitative protein extraction.

Application Information

Western blot 1: 5,000-10,000 with standard ECL
Expected/apparent MW [kDa]: 52.7 kDa (Arabidopsis thaliana), 52.5 kDa (cyanobacteria), 52.3 (Chlamydomonas reinhardtii)
Confirmed reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii, Cyanophora paradoxa, Emiliana huxleyi, Euglena gracilis, Gonyaulax polyedra, Heterosigma akashiwo, Micromonas pusila, Porphyra sp. Spinacia oleracea, Synechococcus PCC 7942, Thalassiosira pseudonana
Predicted reactivity:di and monocots, conifers, mosses, liverworts, welwitschia, green algae, red alge, brown algae, cryptomonad, cyanobacteria including prochlorophytes, gamma-proeobacteria, beta-proteobacteria, alpha proteobacteria
Not reactive in: no confirmed exceptin from predicted reactivity known in the moment
RbcL protein standard: at 0.15 pmoles/®µl, amount enough for generation of standard curve in 85 assays (standard curve: 0.0625 pmoles, 0.125 pmoles, 0.25 pmoles)
PEB extraction buffer: amount enought for 48 isolations of plant material (using 500®µl 1 x PEB for 100 mg fresh weight) or 120 isolations of algal material (using 200®µl 1 x PEB for cell amounts corresponding to 4-10 ug total chlorophyll)