Glutathione Sepharose® 4B
- Reduced glutathione (GSH) covalently coupled to Sepharose® via 10-carbon spacer arm.
- For affinity purification of proteins containing glutathione S-transferase (GST) fusion tag.
- Supplied as suspension in 20% ethanol.
- 1ml drained gel binds more than 5mg of recombinant GST-protein.
- Ligand coupled: Reduced glutathione (GSH)
- Ligand couping method: Epoxy activation
- Matrix: 4 % agarose bead
- Binding capacity: > 5mg GST/ml drained gel
- Exclusion limit: approximately 2 x 107
- Particle size (range): 50-175µm
- Storage: 2-8ºC
Made with SEPHAROSE® chromatography media. SEPHAROSE® is a trademark of GE Healthcare Bio-Sciences AB (formerly known as Amersham Biosciences AB.)
Ref : The Jour. of Biol. Chem. Vol. 281, NO. 31, pp. 21884-21891, August 4, 2006
Storage
: 2-8°CBrand Name
: BIOPLUSSize
: 100 mLCatalog No
: 20182003-5 ( 506407 )